Hotspot

trnS-GCU-ycf12

LSC · 7866-8448 · 583 bp

Back to Nephrolepis

Pi 0.108
MarkerSeek score 83.90
Primer available yes
Label trnS-GCU-ycf12

Feature Metadata

label_name
trnS-GCU-ycf12
feature_type
igs
region
LSC
length_bp
583
variable_sites
62
pi
0.108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-ycf12_p1 GCAATCCGACGCTTTAAACC TGATGCAACTACCAAAGCCA 50.0 45.0 58.1 57.7 665-673 bp 1.0 85.3
trnS-GCU-ycf12_p2 CAATCCGACGCTTTAAACCG TGATGCAACTACCAAAGCCA 50.0 45.0 57.8 57.7 664-672 bp 1.0 85.0
trnS-GCU-ycf12_p3 GCAATCCGACGCTTTAAACC TGCAACTACCAAAGCCAAGA 50.0 45.0 58.1 57.6 662-670 bp 1.0 84.8
trnS-GCU-ycf12_p4 CAATCCGACGCTTTAAACCG TGCAACTACCAAAGCCAAGA 50.0 45.0 57.8 57.6 661-669 bp 1.0 84.5
trnS-GCU-ycf12_p5 GCAATCCGACGCTTTAAACC GGTCCTGATGCAACTACCAA 50.0 50.0 58.1 57.5 670-678 bp 1.0 83.6