Hotspot

matK-ycf94

LSC · 2911-3353 · 443 bp

Back to Nephrolepis

Pi 0.101

MarkerSeek score 77.30

Primer available yes

Label matK-ycf94

Feature Metadata

label_name: matK-ycf94
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 44
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-ycf94_p1	CTTGGTAACGGACGCTATCA	TCGGAGGGACTACTGGTTTT	50.0	50.0	57.4	58.0	778-779 bp	1.0	83.5
matK-ycf94_p2	CTTGGTAACGGACGCTATCA	TTCGGAGGGACTACTGGTTT	50.0	50.0	57.4	58.0	779-780 bp	1.0	83.5
matK-ycf94_p3	CTTGGTAACGGACGCTATCA	TTTCGGAGGGACTACTGGTT	50.0	50.0	57.4	58.0	780-781 bp	1.0	83.5
matK-ycf94_p4	CTTGGTAACGGACGCTATCA	TCAACAGCAACAGTTCGTGA	50.0	45.0	57.4	58.0	981-982 bp	1.0	83.0
matK-ycf94_p5	CTTGGTAACGGACGCTATCA	AGCCGCATGAATCTAACGTT	50.0	45.0	57.4	58.0	1207 bp	0.5	55.2