Hotspot

petB

LSC · 78038-79487 · 1450 bp

Back to Nephelium

Pi 0.00554

MarkerSeek score 62.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1450
variable_sites: 8
pi: 0.00554
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCGACCGCGAAATTTTTGTG	ATTGTTCCTCCCCCATGTAA	45.0	45.0	58.0	56.1	1583-1588 bp	1.0	65.1
petB_p2	TCGACCGCGAAATTTTTGTG	TTATTGTTCCTCCCCCATGT	45.0	45.0	58.0	56.1	1585-1590 bp	1.0	65.1
petB_p3	TCGACCGCGAAATTTTTGTG	TTGTTCCTCCCCCATGTAAT	45.0	45.0	58.0	56.1	1582-1587 bp	1.0	65.1
petB_p4	CGACCGCGAAATTTTTGTGT	ATTGTTCCTCCCCCATGTAA	45.0	45.0	58.3	56.1	1582-1587 bp	1.0	63.3
petB_p5	CGACCGCGAAATTTTTGTGT	TTGTTCCTCCCCCATGTAAT	45.0	45.0	58.3	56.1	1581-1586 bp	1.0	63.3