Hotspot

trnR(ACG)-trnN(GTT)

IRb · 111902-112542 · 641 bp

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Pi 0.00473

MarkerSeek score 62.50

Primer available yes

Label trnR(ACG)-trnN(GTT)

Feature Metadata

label_name: trnR(ACG)-trnN(GTT)
feature_type: igs
region: IRb
length_bp: 641
variable_sites: 3
pi: 0.00473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ACG)-trnN(GTT)_p1	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	739-746 bp	1.0	78.1
trnR(ACG)-trnN(GTT)_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	726-733 bp	1.0	78.1
trnR(ACG)-trnN(GTT)_p3	CAGAGGATTAGAGCACGTGG	TAGAATCTCCCGCTGTTCCT	55.0	50.0	57.8	57.8	784-791 bp	1.0	78.0
trnR(ACG)-trnN(GTT)_p4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	727-734 bp	1.0	77.7
trnR(ACG)-trnN(GTT)_p5	GAACAGATCCAGTGGAGACG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.7	57.8	776-783 bp	1.0	77.5