Hotspot

rpl20-rps12

LSC · 71964-72689 · 726 bp

Back to Nephelium

Pi 0.0069

MarkerSeek score 62.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 5
pi: 0.0069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCGCGAAAACTTGATGCAAA	AGAAACGTCACGAAATCCCC	45.0	50.0	58.2	58.2	872-879 bp	1.0	77.1
rpl20-rps12_p2	CCGCGAAAACTTGATGCAAA	GAGGAACATGTACGAGGGTG	45.0	55.0	58.2	57.7	826-833 bp	1.0	76.3
rpl20-rps12_p3	CCGCGAAAACTTGATGCAAA	AGGAACATGTACGAGGGTGT	45.0	50.0	58.2	58.4	825-832 bp	1.0	75.8
rpl20-rps12_p4	TAAGTCTTGAATGAGCCCCG	AGAAACGTCACGAAATCCCC	50.0	50.0	57.3	58.2	889-896 bp	1.0	73.4
rpl20-rps12_p5	CCGCGAAAACTTGATGCAAA	AGAAACCCAAGACAGCCAAT	45.0	45.0	58.2	57.3	893-900 bp	1.0	73.2