Hotspot

psbE-petL

LSC · 67430-68775 · 1346 bp

Back to Nephelium

Pi 0.00747

MarkerSeek score 63.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 10
pi: 0.00747
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1510-1552 bp	1.0	63.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CGAGGTTATAGTTAAAGCCGC	50.0	47.6	57.9	56.9	1507-1549 bp	1.0	63.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	57.8	57.9	1433-1475 bp	1.0	63.1
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGCCGAGGTTATAGTTAAAGCC	45.0	45.5	58.2	57.9	1519-1561 bp	1.0	63.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	58.2	57.9	1524-1566 bp	1.0	62.9