Hotspot

accD-psaI

LSC · 61624-62352 · 729 bp

Back to Nephelium

Pi 0.00704

MarkerSeek score 62.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 5
pi: 0.00704
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAATCGTACCACGTAACC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	877-896 bp	1.0	76.7
accD-psaI_p2	CCAATCGTACCACGTAACCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	876-895 bp	1.0	76.7
accD-psaI_p3	TCCAATCGTACCACGTAACC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.3	57.9	883-902 bp	1.0	76.1
accD-psaI_p4	CCAATCGTACCACGTAACCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.3	57.9	882-901 bp	1.0	76.0
accD-psaI_p5	CCAATCGTACCACGTAACCTT	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.0	58.0	876-895 bp	1.0	73.7