Hotspot

trnT(TGT)-trnL(TAA)

LSC · 48867-49774 · 908 bp

Back to Nephelium

Pi 0.00572
MarkerSeek score 65.60
Primer available yes
Label trnT(TGT)-trnL(TAA)

Feature Metadata

label_name
trnT(TGT)-trnL(TAA)
feature_type
igs
region
LSC
length_bp
908
variable_sites
5
pi
0.00572
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(TGT)-trnL(TAA)_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 934-958 bp 1.0 82.2
trnT(TGT)-trnL(TAA)_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 942-966 bp 1.0 81.7
trnT(TGT)-trnL(TAA)_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 966-990 bp 1.0 81.2
trnT(TGT)-trnL(TAA)_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 974-998 bp 1.0 80.7
trnT(TGT)-trnL(TAA)_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 948-972 bp 1.0 80.3