Hotspot

psbZ-trnG(GCC)

LSC · 38391-38948 · 558 bp

Back to Nephelium

Pi 0.0108

MarkerSeek score 56.10

Primer available yes

Label psbZ-trnG(GCC)

Feature Metadata

label_name: psbZ-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 6
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG(GCC)_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	712 bp	1.0	79.3
psbZ-trnG(GCC)_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	715 bp	1.0	77.8
psbZ-trnG(GCC)_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	708 bp	1.0	77.6
psbZ-trnG(GCC)_p4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	713 bp	1.0	77.4
psbZ-trnG(GCC)_p5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	711 bp	1.0	76.5