Hotspot

psbC-trnS(TGA)

LSC · 37520-37757 · 238 bp

Back to Nephelium

Pi 0.0336

MarkerSeek score 71.80

Primer available yes

Label psbC-trnS(TGA)

Feature Metadata

label_name: psbC-trnS(TGA)
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 8
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS(TGA)_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	388 bp	1.0	78.2
psbC-trnS(TGA)_p2	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	422 bp	1.0	77.7
psbC-trnS(TGA)_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	387 bp	1.0	77.6
psbC-trnS(TGA)_p4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	399 bp	1.0	77.6
psbC-trnS(TGA)_p5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.4	58.1	433 bp	1.0	77.1