Hotspot

ycf1

SSC · 121491-127082 · 5592 bp

Back to Nepeta

Pi 0.0241
MarkerSeek score 58.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5592
variable_sites
415
pi
0.0241
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CAGGCCTCTGCTATCCCTAT TCGGTTGTGTAATGACGCTT 55.0 45.0 58.1 57.8 975 bp 0.142857 44.1
ycf1_p2 CTATCCCAGGCCTCTGCTAT TCGGTTGTGTAATGACGCTT 55.0 45.0 58.1 57.8 981 bp 0.142857 44.1
ycf1_p3 TATCCCAGGCCTCTGCTATC TCGGTTGTGTAATGACGCTT 55.0 45.0 58.1 57.8 980 bp 0.142857 44.0
ycf1_p4 GCAAGTGGAATGCTATCCCA TCGGTTGTGTAATGACGCTT 50.0 45.0 57.9 57.8 993 bp 0.142857 44.0
ycf1_p5 TTTGCCCTTTCTTGAACGGA TCGGTTGTGTAATGACGCTT 45.0 45.0 57.9 57.8 571 bp 0.142857 44.0