Hotspot

rps15-ycf1

SSC · 121114-121490 · 377 bp

Back to Nepeta

Pi 0.0377
MarkerSeek score 71.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
377
variable_sites
42
pi
0.0377
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TTCCTAGCTGTTCAGGTCCA GAACGTACCAAAAAGCAGCC 50.0 50.0 58.0 58.0 1222-1235 bp 1.0 85.8
rps15-ycf1_p2 AGTCGTTGACGTTTTCCCAA TACCAAAAAGCAGCCGAGTT 45.0 45.0 58.0 58.0 762-771 bp 0.857143 81.5
rps15-ycf1_p3 TTCCTAGCTGTTCAGGTCCA TACCAAAAAGCAGCCGAGTT 50.0 45.0 58.0 58.0 1217-1230 bp 0.857143 79.5
rps15-ycf1_p4 AGTCGTTGACGTTTTCCCAA TGGGACGCAACTTTGAATCA 45.0 45.0 58.0 58.0 1270-1279 bp 0.857143 79.5
rps15-ycf1_p5 TTCCTAGCTGTTCAGGTCCA TGGGACGCAACTTTGAATCA 50.0 45.0 58.0 58.0 1725-1734 bp 0.857143 78.6