Hotspot

rpl32-trnL-UAG

SSC · 111191-111891 · 701 bp

Back to Nepeta

Pi 0.0425
MarkerSeek score 80.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
701
variable_sites
82
pi
0.0425
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCCACATACGACGAAGATT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1466-1539 bp 0.857143 82.5
rpl32-trnL-UAG_p2 GCCCACATACGACGAAGATT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.1 57.8 1465-1538 bp 0.857143 81.9
rpl32-trnL-UAG_p3 GCCCACATACGACGAAGATT CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.1 57.8 1464-1537 bp 0.857143 81.9
rpl32-trnL-UAG_p4 AAAAGCCCACATACGACGAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1470-1543 bp 0.857143 80.2
rpl32-trnL-UAG_p5 GCCCACATACGACGAAGATT AGCAGCGTGTCTACCAATTT 50.0 45.0 58.1 57.8 1459-1532 bp 0.714286 75.5