Hotspot

petG-trnW-CCA

LSC · 65355-65469 · 115 bp

Back to Nepeta

Pi 0.0469
MarkerSeek score 74.50
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
115
variable_sites
12
pi
0.0469
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 GCGATCAGTTGGACCTTTGA AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.2 58.0 182-188 bp 1.0 87.3
petG-trnW-CCA_p2 GCGATCAGTTGGACCTTTGA CAAAACCCGATGTCGTAGGT 50.0 50.0 58.2 57.9 168-174 bp 1.0 87.3
petG-trnW-CCA_p3 GCGATCAGTTGGACCTTTGA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.2 58.0 204-210 bp 1.0 86.8
petG-trnW-CCA_p4 GCGATCAGTTGGACCTTTGA CCCGATGTCGTAGGTTCAAA 50.0 50.0 58.2 57.6 163-169 bp 1.0 85.2
petG-trnW-CCA_p5 GCGATCAGTTGGACCTTTGA CGCTCTTAGTTCAGTTCGGT 50.0 50.0 58.2 57.7 202-208 bp 1.0 84.2