Pi
0.019
MarkerSeek score
61.70
Primer available
yes
Label
ndhC-trnM-CAU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhC-trnM-CAU_p1 | CTAGAAAGTTTCTCCGGCCC | TGATGCGGAAAAGGGTAGTG | 55.0 | 50.0 | 58.0 | 57.9 | 2336-2364 bp | 1.0 | 84.8 |
| ndhC-trnM-CAU_p2 | CTAGAAAGTTTCTCCGGCCC | GGCAAATCTAGCTCTCCGAC | 55.0 | 55.0 | 58.0 | 58.1 | 2231-2259 bp | 1.0 | 84.7 |
| ndhC-trnM-CAU_p3 | CTAGAAAGTTTCTCCGGCCC | GAGGCAAATCTAGCTCTCCG | 55.0 | 55.0 | 58.0 | 57.9 | 2233-2261 bp | 1.0 | 84.7 |
| ndhC-trnM-CAU_p4 | CTAGAAAGTTTCTCCGGCCC | ATGGGCGGTTTTGCTAGAAT | 55.0 | 45.0 | 58.0 | 57.9 | 2386-2414 bp | 1.0 | 84.5 |
| ndhC-trnM-CAU_p5 | CTAGAAAGTTTCTCCGGCCC | CGCCTTAAGGACCAATGGTT | 55.0 | 50.0 | 58.0 | 58.2 | 2419-2447 bp | 1.0 | 84.2 |