Hotspot

rps4-trnT-UGU

LSC · 45397-45782 · 386 bp

Back to Nepeta

Pi 0.0397
MarkerSeek score 78.10
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
386
variable_sites
38
pi
0.0397
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TCTAAGATCACTCCCGGCTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 503-595 bp 1.0 87.2
rps4-trnT-UGU_p2 TTCTAAGATCACTCCCGGCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 504-596 bp 1.0 87.2
rps4-trnT-UGU_p3 TCTAAGATCACTCCCGGCTT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.8 57.8 471-563 bp 1.0 86.6
rps4-trnT-UGU_p4 TTCTAAGATCACTCCCGGCT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.8 57.8 472-564 bp 1.0 86.6
rps4-trnT-UGU_p5 GTAAGTCCTGGTAAAGCCCC CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.6 57.9 469-561 bp 1.0 85.5