Hotspot

rps16-trnQ-UUG

LSC · 5849-6853 · 1005 bp

Back to Nepeta

Pi 0.0388
MarkerSeek score 78.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1005
variable_sites
111
pi
0.0388
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCCTAGATCCTTGCCTCTGA GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 1178-1306 bp 1.0 90.4
rps16-trnQ-UUG_p2 TCGCACGTTGCTTTCTATCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 1075 bp 0.142857 44.5
rps16-trnQ-UUG_p3 AGTCGCACGTTGCTTTCTAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 1077 bp 0.142857 44.5
rps16-trnQ-UUG_p4 TCGCACGTTGCTTTCTATCA GTCTCGCTATTCGGAGGTTC 45.0 55.0 57.9 57.9 1088 bp 0.142857 44.1
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAT GTCTCGCTATTCGGAGGTTC 45.0 55.0 57.9 57.9 1090 bp 0.142857 44.1