Hotspot

trnH-GUG-psbA

LSC · 83-339 · 257 bp

Back to Nepeta

Pi 0.0681
MarkerSeek score 73.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
257
variable_sites
37
pi
0.0681
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.90
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTGTTGCGTATTCAGCTCCT 55.0 50.0 58.1 58.0 885-1016 bp 1.0 86.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GAGTGGGAGCTTAGTTTCCG 55.0 55.0 58.1 58.0 928-1059 bp 1.0 86.6
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 942-1073 bp 1.0 86.5
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 941-1072 bp 1.0 86.5
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 943-1074 bp 1.0 86.5