Hotspot

ccsA-ndhD

SSC · 143360-143590 · 231 bp

Back to Nepenthes

Pi 0.0649

MarkerSeek score 68.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 15
pi: 0.0649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	TACAGGACTCAATGGAGCCA	45.0	50.0	58.0	58.0	1303-1311 bp	1.0	80.7
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	TACAGGACTCAATGGAGCCA	45.0	50.0	58.0	58.0	1302-1310 bp	1.0	80.7
ccsA-ndhD_p3	ATGCAGCTCTTTTATGCGGA	ACCAGGCATGAGTGGTTTTT	45.0	45.0	58.0	57.9	1114-1122 bp	1.0	80.6
ccsA-ndhD_p4	TGCAGCTCTTTTATGCGGAT	ACCAGGCATGAGTGGTTTTT	45.0	45.0	58.0	57.9	1113-1121 bp	1.0	80.6
ccsA-ndhD_p5	ACCCAAGGCTTTGCTACTTC	TACAGGACTCAATGGAGCCA	50.0	50.0	58.1	58.0	1424-1432 bp	1.0	80.3