Hotspot

rpl16-rps3

LSC · 110095-111211 · 1117 bp

Back to Nepenthes

Pi 0.0162

MarkerSeek score 68.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 18
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	CCCTACAAACCATTCGAGCT	50.0	50.0	58.4	57.6	1337-1339 bp	1.0	75.1
rpl16-rps3_p2	GGGTTCAAGTGCCTGAAGAG	CCCTACAAACCATTCGAGCT	55.0	50.0	58.5	57.6	1339-1341 bp	1.0	74.6
rpl16-rps3_p3	TTCAAGTGCCTGAAGAGCAT	CCCTACAAACCATTCGAGCT	45.0	50.0	57.4	57.6	1336-1338 bp	1.0	73.7
rpl16-rps3_p4	GTTCAAGTGCCTGAAGAGCA	GGGTTCCCCTACAAACCATT	50.0	50.0	58.4	57.4	1343-1345 bp	1.0	73.4
rpl16-rps3_p5	GTTCAAGTGCCTGAAGAGCA	CCCCTACAAACCATTCGAGC	50.0	55.0	58.4	58.6	1338-1340 bp	1.0	73.4