Hotspot

trnR-UCU-atpA

LSC · 35178-35512 · 335 bp

Back to Nepenthes

Pi 0.0403

MarkerSeek score 67.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 12
pi: 0.0403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	430-432 bp	1.0	72.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCAGAAGC	47.6	50.0	57.3	57.8	436-438 bp	1.0	71.2
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	GAGGAAGCAGAAGCCCTTTT	42.9	50.0	56.6	58.1	429-431 bp	1.0	66.4
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TCAAGAACAGATGGAACGCT	47.6	45.0	57.3	57.2	398-400 bp	1.0	66.1
trnR-UCU-atpA_p5	GTTCAAATCCTATTGGACGCA	TTCACTGAGGAAGCAGAAGC	42.9	50.0	56.6	57.8	435-437 bp	1.0	65.6