Hotspot

trnS-GCU-trnS-CGA

LSC · 33091-34197 · 1107 bp

Back to Nepenthes

Pi 0.0197

MarkerSeek score 68.10

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 21
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	ACGGAAAGAGAGGGATTCGA	CGAAGGACCCCTTAACTCTT	50.0	50.0	58.2	56.6	1232-1264 bp	1.0	71.4
trnS-GCU-trnS-CGA_p2	ACGAAAACGGAAAGAGAGGG	CGAAGGACCCCTTAACTCTT	50.0	50.0	57.8	56.6	1238-1270 bp	1.0	71.3
trnS-GCU-trnS-CGA_p3	CGAATGACTCGTACAACGGA	CGAAGGACCCCTTAACTCTT	50.0	50.0	57.7	56.6	1202-1234 bp	1.0	70.7
trnS-GCU-trnS-CGA_p4	CGAAAACGGAAAGAGAGGGA	CGAAGGACCCCTTAACTCTT	50.0	50.0	57.5	56.6	1237-1269 bp	1.0	69.0
trnS-GCU-trnS-CGA_p5	CTTTCGTCCACTCAGCCATC	CGAAGGACCCCTTAACTCTT	55.0	50.0	58.6	56.6	1168-1200 bp	1.0	67.7