Hotspot

clpP-psbB

LSC · 42164-42793 · 630 bp

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Pi 0.096

MarkerSeek score 88.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 120
pi: 0.096
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	672-1047 bp	1.0	87.7
clpP-psbB_p2	TTTGGAACACCAATGGGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.3	58.2	670-1045 bp	1.0	86.8
clpP-psbB_p3	TCCAGGACTCCGAAAAGGTA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	692-1067 bp	1.0	86.4
clpP-psbB_p4	CCAGGACTCCGAAAAGGTAC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.6	58.2	691-1066 bp	1.0	85.9
clpP-psbB_p5	CTTTTGGAACACCAATGGGC	ACACGATACCAAGGCAAACC	50.0	50.0	57.8	58.5	675-1050 bp	1.0	85.8