Hotspot

trnE-UUC-trnT-GGU

LSC · 14924-15969 · 1046 bp

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Pi 0.0598

MarkerSeek score 86.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 133
pi: 0.0598
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	892-1134 bp	1.0	90.1
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	889-1131 bp	1.0	90.0
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	870-1112 bp	1.0	89.2
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	867-1109 bp	1.0	89.1
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	ACCATGGCGTTACTCTACCA	50.0	50.0	57.8	58.4	893-1135 bp	1.0	87.2