Hotspot

rpoB-trnC-GCA

LSC · 12939-13477 · 539 bp

Back to Neottia

Pi 0.0578

MarkerSeek score 83.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 539
variable_sites: 68
pi: 0.0578
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCGGTCTCCATAGCAAGAAA	TTATAGGTCCAGGCCCTACG	50.0	55.0	58.0	58.0	1290 bp	0.2	47.8
rpoB-trnC-GCA_p2	GCGGTCTCCATAGCAAGAAA	TACTTGTTTGAGCCACACCC	50.0	50.0	58.0	58.0	1345 bp	0.2	47.6
rpoB-trnC-GCA_p3	TCAACAAGTCTACGCGTGAG	TTATAGGTCCAGGCCCTACG	50.0	55.0	58.0	58.0	1355 bp	0.2	47.6
rpoB-trnC-GCA_p4	TCAACAAGTCTACGCGTGAG	TACTTGTTTGAGCCACACCC	50.0	50.0	58.0	58.0	1410 bp	0.2	47.4
rpoB-trnC-GCA_p5	TTCCTTCGGACGTGTCAATC	TTATAGGTCCAGGCCCTACG	50.0	55.0	57.9	58.0	956 bp	0.2	47.4