Hotspot

trnS-GCU-trnG-GCC

LSC · 6834-7375 · 542 bp

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Pi 0.0605

MarkerSeek score 82.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 69
pi: 0.0605
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	TTAGTCCCCTCAGCCATCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	599-862 bp	1.0	61.3
trnS-GCU-trnG-GCC_p2	TTTAGTCCCCTCAGCCATCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	600-863 bp	1.0	60.8
trnS-GCU-trnG-GCC_p3	TTAGTCCCCTCAGCCATCTC	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.2	57.3	602-865 bp	1.0	60.2
trnS-GCU-trnG-GCC_p4	TTAGTCCCCTCAGCCATCTC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.2	57.3	601-864 bp	1.0	60.2
trnS-GCU-trnG-GCC_p5	TTTAGTCCCCTCAGCCATCT	GAACGAATCACACTTTTACCACT	50.0	39.1	57.7	57.3	602-865 bp	1.0	59.7