Hotspot

rps16-trnQ-UUG

LSC · 4645-5480 · 836 bp

Back to Neottia

Pi 0.127

MarkerSeek score 83.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 211
pi: 0.127
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCGGAATTCGTTGTCTTTGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1443-1627 bp	1.0	91.1
rps16-trnQ-UUG_p2	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	933-1129 bp	1.0	90.9
rps16-trnQ-UUG_p3	GGCTCGAGCTCCATCATATG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	1094-1282 bp	0.6	75.3
rps16-trnQ-UUG_p4	CCGGAATTCGTTGTCTTTGC	CCAGGGATAACCGGCATAAC	50.0	55.0	58.0	58.1	2009-2117 bp	0.6	74.4
rps16-trnQ-UUG_p5	GGCTCGAGCTCCATCATATG	CCAGGGATAACCGGCATAAC	55.0	55.0	58.0	58.1	1659-1660 bp	0.4	65.6