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trnK-UUU-rps16

LSC · 2768-3463 · 696 bp

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Pi 0.0967

MarkerSeek score 78.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 124
pi: 0.0967
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GCGCAAAAGATCATAGTCGC	CGCAAAGCAAAGACAACGAA	50.0	45.0	58.0	58.2	1224-1380 bp	0.8	82.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1151-1348 bp	0.8	82.4
trnK-UUU-rps16_p3	GCGCAAAAGATCATAGTCGC	GCAAAGACAACGAATTCCGG	50.0	50.0	58.0	58.0	1218-1374 bp	0.6	75.6
trnK-UUU-rps16_p4	TACCTCATCCAAATGCGCAA	GCAAAGACAACGAATTCCGG	45.0	50.0	57.9	58.0	1388 bp	0.2	46.7
trnK-UUU-rps16_p5	TTACCTCATCCAAATGCGCA	GCAAAGACAACGAATTCCGG	45.0	50.0	57.9	58.0	1389 bp	0.2	46.7