Hotspot

rpl32-trnL-CUA

SSC · 113401-114185 · 785 bp

Back to Neotorularia

Pi 0.0859
MarkerSeek score 80.50
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
785
variable_sites
65
pi
0.0859
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GCACTCTCGTACGGATTTCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 1158-1211 bp 1.0 85.8
rpl32-trnL-CUA_p2 GCACTCTCGTACGGATTTCA TGCAGACTGAGGCATTTTGT 50.0 45.0 57.7 58.0 1714-1752 bp 1.0 84.7
rpl32-trnL-CUA_p3 GCACTCTCGTACGGATTTCA TGGAAAAAGCCCACGAAAGA 50.0 45.0 57.7 57.9 1586-1624 bp 1.0 84.7
rpl32-trnL-CUA_p4 GCACTCTCGTACGGATTTCA TAGGGCCAACTTTGCGAAAT 50.0 45.0 57.7 58.1 1850-1888 bp 1.0 83.8
rpl32-trnL-CUA_p5 GCACTCTCGTACGGATTTCA TAACCACTGGGACTGGAGAG 50.0 55.0 57.7 58.1 1785 bp 0.5 57.3