Hotspot

ndhF-rpl32

SSC · 112407-113241 · 835 bp

Back to Neotorularia

Pi 0.057
MarkerSeek score 81.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
835
variable_sites
45
pi
0.057
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTGGAATGAAAGGGATGATCCA ACGAATACGCTTTTTCGAGA 45.5 40.0 58.1 55.5 909-929 bp 1.0 51.4
ndhF-rpl32_p2 AGTGGAATGAAAGGGATGATCC ACGAATACGCTTTTTCGAGA 45.5 40.0 57.8 55.5 910-930 bp 1.0 50.8
ndhF-rpl32_p3 TGGAATGAAAGGGATGATCCA ACGAATACGCTTTTTCGAGA 42.9 40.0 56.7 55.5 908-928 bp 1.0 49.6
ndhF-rpl32_p4 GTGGAATGAAAGGGATGATCCA ACGAATACGCTTTTTCGAGAT 45.5 38.1 58.1 56.1 909-929 bp 1.0 47.9
ndhF-rpl32_p5 AGTGGAATGAAAGGGATGATCC ACGAATACGCTTTTTCGAGAT 45.5 38.1 57.8 56.1 910-930 bp 1.0 47.8