Hotspot

psaJ-rpl33

LSC · 66745-67160 · 416 bp

Back to Neotorularia

Pi 0.0605

MarkerSeek score 77.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 416
variable_sites: 25
pi: 0.0605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTAGCGCC	ATTCCCGCCGATTCTTTCTT	50.0	45.0	57.8	57.6	619 bp	1.0	79.1
psaJ-rpl33_p2	ACATATCTTTCCGTAGCGCC	AATTCCCGCCGATTCTTTCT	50.0	45.0	57.8	57.6	620 bp	1.0	79.0
psaJ-rpl33_p3	TAGCGCCGGTACTAAGTACT	AATTCCCGCCGATTCTTTCT	50.0	45.0	57.7	57.6	607 bp	1.0	77.7
psaJ-rpl33_p4	TAGCGCCGGTACTAAGTACT	ATTCCCGCCGATTCTTTCTT	50.0	45.0	57.7	57.6	606 bp	1.0	77.7
psaJ-rpl33_p5	GTAGCGCCGGTACTAAGTAC	ATTCCCGCCGATTCTTTCTT	55.0	45.0	57.6	57.6	607 bp	1.0	77.2