Hotspot

trnP-CCA-psaJ

LSC · 66262-66615 · 354 bp

Back to Neotorularia

Pi 0.0484

MarkerSeek score 77.30

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 17
pi: 0.0484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	ACCCGTGACATTTTGTACCC	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	444-455 bp	1.0	81.6
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	443-454 bp	1.0	81.6
trnP-CCA-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	455-466 bp	1.0	81.4
trnP-CCA-psaJ_p4	CAGGATTTGAACCCGTGACA	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	454-465 bp	1.0	81.4
trnP-CCA-psaJ_p5	TCCCTCGTTACTGCTCAGAA	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	496-507 bp	1.0	81.3