Hotspot

petA-psbJ

LSC · 62429-63336 · 908 bp

Back to Neotorularia

Pi 0.0656

MarkerSeek score 76.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 59
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGTGGATGCATCGAATGGAC	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1765-1804 bp	1.0	83.2
petA-psbJ_p2	AGTGGATGCATCGAATGGAC	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1764-1803 bp	1.0	83.2
petA-psbJ_p3	GTTTCAGAGGGCGAATCCAT	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1703-1742 bp	1.0	83.2
petA-psbJ_p4	TTCCTATTCTTGCGCCAGAC	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1964-2003 bp	1.0	83.0
petA-psbJ_p5	TTCCTATTCTTGCGCCAGAC	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1963-2002 bp	1.0	83.0