Hotspot

ndhC-trnV-GUA

LSC · 50187-51052 · 866 bp

Back to Neotorularia

Pi 0.0607
MarkerSeek score 77.50
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
866
variable_sites
52
pi
0.0607
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 GCCCAGAAAATATCATATTCGT GTTCGAGTCCGTATAGCCCT 36.4 55.0 54.4 58.7 922-963 bp 1.0 45.3
ndhC-trnV-GUA_p2 GCCCAGAAAATATCATATTCGT TTCGAGTCCGTATAGCCCTA 36.4 50.0 54.4 57.1 921-962 bp 1.0 45.3
ndhC-trnV-GUA_p3 GCCCAGAAAATATCATATTCGT AGGTCTACGGTTCGAGTCC 36.4 57.9 54.4 58.1 931-972 bp 1.0 45.2
ndhC-trnV-GUA_p4 GCCCAGAAAATATCATATTCGT TCGAACTCTTTTACCGAGCAG 36.4 47.6 54.4 58.1 950-991 bp 1.0 45.1
ndhC-trnV-GUA_p5 GCCCAGAAAATATCATATTCGT TCGAACTCTTTTACCGAGCA 36.4 45.0 54.4 56.9 950-991 bp 1.0 45.1