Hotspot

trnL-UUA-trnF-UUC

LSC · 47368-48058 · 691 bp

Back to Neotorularia

Pi 0.148

MarkerSeek score 81.30

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 102
pi: 0.148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	781-811 bp	1.0	83.9
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	778-808 bp	1.0	80.6
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	770-800 bp	1.0	80.0
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	757-787 bp	1.0	79.5
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TTCAGTCCTCTGCTCTACCA	50.0	50.0	57.5	57.4	758-788 bp	1.0	79.5