Hotspot

trnT-ACA-trnL-UUA

LSC · 45961-46777 · 817 bp

Back to Neotorularia

Pi 0.0659

MarkerSeek score 84.80

Primer available yes

Label trnT-ACA-trnL-UUA

Feature Metadata

label_name: trnT-ACA-trnL-UUA
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 49
pi: 0.0659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACA-trnL-UUA_p1	GCGATGCTCTAACCTCTGAG	CTACCGATTCCGCCATATCC	55.0	55.0	57.9	57.7	869-876 bp	1.0	85.1
trnT-ACA-trnL-UUA_p2	TATGGAAAAGCCGGCTATCG	CTACCGATTCCGCCATATCC	50.0	55.0	57.8	57.7	920-927 bp	1.0	84.6
trnT-ACA-trnL-UUA_p3	GGAATCGAACCGATGACCAT	CTACCGATTCCGCCATATCC	50.0	55.0	57.8	57.7	901-908 bp	1.0	84.0
trnT-ACA-trnL-UUA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	910-917 bp	1.0	83.2
trnT-ACA-trnL-UUA_p5	TATGGAAAAGCCGGCTATCG	GGTTTCCATACCAAGGCTCA	50.0	50.0	57.8	57.5	961-968 bp	1.0	82.7