Hotspot

trnS-UCA-psbZ

LSC · 35164-35509 · 346 bp

Back to Neotorularia

Pi 0.0551

MarkerSeek score 76.70

Primer available yes

Label trnS-UCA-psbZ

Feature Metadata

label_name: trnS-UCA-psbZ
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 19
pi: 0.0551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UCA-psbZ_p1	TTTTCAAGACCGGAGCCATC	ACGCAAATACAACGGGTACA	50.0	45.0	58.2	57.8	471-472 bp	1.0	80.6
trnS-UCA-psbZ_p2	TTTTCAAGACCGGAGCCATC	TACTCGACCAACCATCAGGA	50.0	50.0	58.2	57.8	492-493 bp	1.0	80.0
trnS-UCA-psbZ_p3	TTTTCAAGACCGGAGCCATC	AGGAGACGCAAATACAACGG	50.0	50.0	58.2	58.3	476-477 bp	1.0	79.7
trnS-UCA-psbZ_p4	GTTTTCAAGACCGGAGCCAT	ACGCAAATACAACGGGTACA	50.0	45.0	58.5	57.8	472-473 bp	1.0	78.3
trnS-UCA-psbZ_p5	TTTTCAAGACCGGAGCCATC	CAACCATCAGGAGACGCAAA	50.0	50.0	58.2	58.5	484-485 bp	1.0	78.1