Hotspot

psbM-trnD-GAC

LSC · 28587-29615 · 1029 bp

Back to Neotorularia

Pi 0.0498

MarkerSeek score 86.00

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 47
pi: 0.0498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	TGAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.5	50.0	58.1	58.5	1076-1142 bp	1.0	66.4
psbM-trnD-GAC_p2	GAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	56.4	58.5	1075-1141 bp	1.0	63.1
psbM-trnD-GAC_p3	AGGAATGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1081-1147 bp	1.0	63.0
psbM-trnD-GAC_p4	TGAGAATGAAGAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.5	50.0	58.1	57.0	1077-1143 bp	1.0	62.2
psbM-trnD-GAC_p5	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	1074-1140 bp	1.0	61.0