Hotspot

petN-psbM

LSC · 27951-28481 · 531 bp

Back to Neotorularia

Pi 0.055

MarkerSeek score 80.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 29
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.0	57.0	639-642 bp	1.0	68.3
petN-psbM_p2	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.9	57.0	638-641 bp	1.0	67.7
petN-psbM_p3	CTCTCGTAGTGTGGGGAAGA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	642-645 bp	1.0	67.0
petN-psbM_p4	CTCTCTCGTAGTGTGGGGAA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	644-647 bp	1.0	67.0
petN-psbM_p5	TCTCTCGTAGTGTGGGGAAG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	643-646 bp	1.0	67.0