Hotspot

atpF-atpH

LSC · 12532-12996 · 465 bp

Back to Neotorularia

Pi 0.0495

MarkerSeek score 75.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 22
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GAAAGAATCGGTTAAATTTTTCA	TGGCCTGGTTGTAGCATTAG	26.1	50.0	52.3	57.6	532-538 bp	1.0	46.4
atpF-atpH_p2	GAAAGAATCGGTTAAATTTTTCAT	TGGCCTGGTTGTAGCATTAG	25.0	50.0	52.9	57.6	532-538 bp	1.0	46.4
atpF-atpH_p3	GAAAGAATCGGTTAAATTTTTCA	TTTATGGCCTGGTTGTAGCA	26.1	45.0	52.3	57.1	536-542 bp	1.0	46.4
atpF-atpH_p4	GAAAGAATCGGTTAAATTTTTCA	GGCCTGGTTGTAGCATTAGC	26.1	55.0	52.3	59.0	531-537 bp	1.0	46.4
atpF-atpH_p5	GAAAGAATCGGTTAAATTTTTCA	ATGGCCTGGTTGTAGCATTAG	26.1	47.6	52.3	58.1	533-539 bp	1.0	46.4