Hotspot

trnG-GGA-trnR-AGA

LSC · 9140-9300 · 161 bp

Back to Neotorularia

Pi 0.0807

MarkerSeek score 75.40

Primer available yes

Label trnG-GGA-trnR-AGA

Feature Metadata

label_name: trnG-GGA-trnR-AGA
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 13
pi: 0.0807
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GGA-trnR-AGA_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	283-290 bp	1.0	50.8
trnG-GGA-trnR-AGA_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	289-296 bp	1.0	50.7
trnG-GGA-trnR-AGA_p3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	303-310 bp	1.0	50.3
trnG-GGA-trnR-AGA_p4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	291-298 bp	1.0	50.3
trnG-GGA-trnR-AGA_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	284-291 bp	1.0	49.8