Hotspot

rps16-trnQ-CAA

LSC · 6157-6523 · 367 bp

Back to Neotorularia

Pi 0.0656

MarkerSeek score 75.50

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 24
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	439-440 bp	1.0	82.8
rps16-trnQ-CAA_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	449-450 bp	1.0	82.0
rps16-trnQ-CAA_p3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	465-466 bp	1.0	80.4
rps16-trnQ-CAA_p4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	466-467 bp	1.0	80.3
rps16-trnQ-CAA_p5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	467-468 bp	1.0	78.6