Hotspot

trnG-UCC

LSC · 13155-13202 · 48 bp

Back to Neomicrocalamus

Pi 0.0208

MarkerSeek score 63.40

Primer available yes

Label trnG-UCC

Feature Metadata

label_name: trnG-UCC
feature_type: tRNA
region: LSC
length_bp: 48
variable_sites: 1
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	CAAACTGCTCTACTCCGCTC	AAGGAGCCGAATGAAATCCA	55.0	45.0	58.4	57.2	238 bp	1.0	71.0
trnG-UCC_p2	CAAACTGCTCTACTCCGCTC	AGGAGCCGAATGAAATCCAA	55.0	45.0	58.4	57.2	237 bp	1.0	71.0
trnG-UCC_p3	TGGTGGACGAAAAAGGTTGA	AAGGAGCCGAATGAAATCCA	45.0	45.0	57.6	57.2	203 bp	1.0	70.6
trnG-UCC_p4	TGGTGGACGAAAAAGGTTGA	AGGAGCCGAATGAAATCCAA	45.0	45.0	57.6	57.2	202 bp	1.0	70.6
trnG-UCC_p5	ACTGGTGGACGAAAAAGGTT	AGGAGCCGAATGAAATCCAA	45.0	45.0	57.6	57.2	204 bp	1.0	70.5