Hotspot

rps3-rpl22

LSC · 82198-82257 · 60 bp

Back to Neomicrocalamus

Pi 0.0339

MarkerSeek score 73.50

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.9	57.7	228-229 bp	1.0	78.2
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.8	57.7	194-195 bp	1.0	77.0
rps3-rpl22_p3	CCAAGTCTGAAACCGAGTGG	AAATTCAGACCTCGAGCTCG	55.0	50.0	58.5	57.7	193-194 bp	1.0	75.1
rps3-rpl22_p4	CAAGTCTGAAACCGAGTGGA	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.5	57.7	192-193 bp	1.0	74.7
rps3-rpl22_p5	GTTGTGCGAACCAAAAGGAA	AAATTCAGACCTCGAGCTCG	45.0	50.0	57.4	57.7	231-232 bp	1.0	74.2