Hotspot

trnC-GCA-rpoB

LSC · 20144-21302 · 1159 bp

Back to Neomicrocalamus

Pi 0.0138

MarkerSeek score 64.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 16
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1243-1254 bp	1.0	77.9
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1242-1253 bp	1.0	77.9
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1307-1318 bp	1.0	77.4
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1306-1317 bp	1.0	77.3
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1308-1319 bp	1.0	77.3