Hotspot

psbM-petN

LSC · 18341-19076 · 736 bp

Back to Neomicrocalamus

Pi 0.0109

MarkerSeek score 65.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 8
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	898 bp	1.0	68.2
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	42.9	60.0	57.6	58.3	842 bp	1.0	67.2
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	42.9	55.0	57.6	58.4	841 bp	1.0	66.5
psbM-petN_p4	TGCTACTGCACTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	56.1	58.2	898 bp	1.0	64.1
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	AGTAGTATGGGGGAGGAGTG	42.9	55.0	57.6	57.2	843 bp	1.0	63.3