Hotspot

psbZ-trnfM-CAU

LSC · 12191-12990 · 800 bp

Back to Neomicrocalamus

Pi 0.0102

MarkerSeek score 65.80

Primer available yes

Label psbZ-trnfM-CAU

Feature Metadata

label_name: psbZ-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 8
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnfM-CAU_p1	TTGCTTCTCCTGATGGTTGG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.8	57.8	948-957 bp	1.0	78.4
psbZ-trnfM-CAU_p2	GCTTCTCCTGATGGTTGGTC	CGAGGCTCATAACCTTGAGG	55.0	55.0	58.3	57.8	946-955 bp	1.0	78.0
psbZ-trnfM-CAU_p3	TTGCTTCTCCTGATGGTTGG	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.8	58.3	963-972 bp	1.0	78.0
psbZ-trnfM-CAU_p4	GCTTCTCCTGATGGTTGGTC	CAGTTTGGTAGCTCACGAGG	55.0	55.0	58.3	58.3	961-970 bp	1.0	77.6
psbZ-trnfM-CAU_p5	TTGCTTCTCCTGATGGTTGG	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	57.8	58.4	976-985 bp	1.0	77.3