Hotspot

rpl2

LSC · 87918-89417 · 1500 bp

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Pi 0

MarkerSeek score 26.60

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GGAATGCCCACGAATCTGAT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2624-2669 bp	1.0	80.7
rpl2_p2	ATAATGGTAGATGCCCGGGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2264-2309 bp	1.0	80.7
rpl2_p3	ATAATGGTAGATGCCCGGGA	TGAATTTGGGTCACTTGCGA	50.0	45.0	58.0	58.0	2316-2361 bp	1.0	80.6
rpl2_p4	GGAATGCCCACGAATCTGAT	TGAATTTGGGTCACTTGCGA	50.0	45.0	58.0	58.0	2676-2721 bp	1.0	80.5
rpl2_p5	GGAATGCCCACGAATCTGAT	CATCAACTACCCGGGAAAGG	50.0	55.0	58.0	58.0	2216-2220 bp	1.0	79.8