Hotspot

ndhF

SSC · 113878-116106 · 2229 bp

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Pi 0.00105

MarkerSeek score 44.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 7
pi: 0.00105
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.0	58.3	2882-2886 bp	1.0	77.6
ndhF_p2	ACAGGAACAAGAGGGATCCA	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.0	58.3	2868-2872 bp	1.0	77.4
ndhF_p3	AAGAGGGATCCACCGAAGAA	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.0	58.3	2860-2864 bp	1.0	77.4
ndhF_p4	ACGAAGGGGACTAAACAGGA	ATAAGGGCTGGTGAATGGGA	50.0	50.0	58.0	58.4	2873-2877 bp	1.0	77.0
ndhF_p5	TTTAGGCAGAATACCGTCGC	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.1	58.3	2997 bp	0.833333	70.5