Hotspot

rpl32-trnL-UAG

SSC · 117417-118768 · 1352 bp

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Pi 0.00173
MarkerSeek score 60.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1352
variable_sites
7
pi
0.00173
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGCGGCAAAAGCTTTATCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 1482-1486 bp 1.0 77.5
rpl32-trnL-UAG_p2 CGCGGCAAAAGCTTTATCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 1475-1479 bp 1.0 77.0
rpl32-trnL-UAG_p3 CGCGGCAAAAGCTTTATCTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.7 57.8 1481-1485 bp 1.0 76.9
rpl32-trnL-UAG_p4 CGCGGCAAAAGCTTTATCTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.7 57.8 1480-1484 bp 1.0 76.9
rpl32-trnL-UAG_p5 CGCGGCAAAAGCTTTATCTT GAGCAGCGTGTCTACCAATT 45.0 50.0 57.7 58.3 1476-1480 bp 1.0 76.3